Description
Our complete set of services for protein expression include gene synthesis (amplification) and subcloning from a variety of sources to create optimized vectors containing appropriate N-terminal or C-terminal tags, transfection or transformation of the recombinant constructs into appropriate cell lines, rigorous testing to identify cells expressing the target protein, large-scale cell culture (production), and final recombinant protein purification using appropriate tag-specific affinity columns. We use a number of protein expression systems, including bacterial cells (E. coli with PET vector), insect cells (Sf9 cells with pIB/V5-Histadine vector) and mammalian cells (HEK 293 cells with pLOC vector). We can help to design and use the method that is best for the type of antibody production needed. More importantly, we have the world's largest cDNA collection, which provides us immediate access to source coding sequence to construct vectors very quickly and accurately.